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amp activated protein kinase α ampkα  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc amp activated protein kinase α ampkα
    Amp Activated Protein Kinase α Ampkα, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 4498 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ampk%CE%B1/AMPKalpha+Antibody/pmc12811471-130-16-22
    Average 97 stars, based on 4498 article reviews
    amp activated protein kinase α ampkα - by Bioz Stars, 2026-10
    97/100 stars

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    other:

    Article Title: AMPK inhibition and elevated angiogenin are associated with tRNA fragmentation in the male germline exposed to a high-fat diet
    Article Snippet: , AMPKα , Cell signaling technology , 2532 , 1:1000 in BSA.

    Article Title: Neuronal PPP2R5C in plasma is a potential biomarker for early diagnosis of Alzheimer’s disease
    Article Snippet: AMPKα , Cell Signaling Technology, #2603 , RRID: AB_490795.

    Expressing:

    Article Title: Enhancing autophagy or increasing longevity by administration of urolithins
    Article Snippet: .. Protein expression levels were examined for autophagy-related proteins LC3-I and LC3-II (Cell Signaling antibody #4108), p62 (Cell Signaling antibody #5114), AMPKα (Cell Signaling antibody #2603), and p-AMPKα (Cell Signaling antibody #2531). .. The housekeeping protein, B-actin (Cell Signaling Antibody #4967) level was measured as a loading control.

    Article Title: Enhancing autophagy or increasing longevity by administration of urolithins
    Article Snippet: .. Protein expression levels were examined for autophagy related proteins LC3-I and LC3-II (Cell Signaling antibody #4108), p62 (Cell Signalling antibody #5114), AMPKα (Cell Signaling antibody #2603), and p-AMPKα (Cell Signaling antibody #2531). ..

    Membrane:

    Article Title: Metformin drives HIF-1α–mediated dual metabolic reprogramming to enhance γδ T cell therapy in triple-negative breast cancer
    Article Snippet: Samples were resolved on 10% SDS–polyacrylamide gels and electrotransferred to 0.45-μm nitrocellulose membranes (IPVH00010, Merck millipore). .. The membrane was blocked with 5% non-fat milk in TBST (JXF003, Jingxin Bio) for 1 h at room temperature and subsequently incubated overnight at 4 °C with primary antibodies targeting phospho-AMPKα (2535 T, CST), AMPKα (2793S, CST), HIF-1α (2178S, CST), and β-actin (4970S, CST). .. The membrane was then subjected to three consecutive washes with TBST for 5 min, incubated for 1 h at room temperature with Anti-rabbit IgG HRP-linked Antibody (7074S, CST) or goat anti-mouse IgG HRP (abs20039, absin), washed three times in TBST for 5 min, and detected using the Omni-ECLTM Femto Light Chemiluminescence Kit (SQ201, EpiZyme).

    Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice
    Article Snippet: Densitometry was analyzed using ImageLab software (V6.0.1; Bio-Rad). .. Immunoblotting was performed with antibodies against lysocardiolipin acyltransferase 1(LCLAT1, PA5-25627; Thermo Fisher Scientific), PGC-1α (ab191838; Abcam, Cambridge, UK), MFN2 (PA5-118059; Thermo Fisher Scientific), ANP (sc-18811; Santa Cruz, Dallas, TX, USA), tumor necrosis factor alpha (TNFα, 3707; Cell Signaling, Danvers, MA, USA), phosphorylated adenosine monophosphate-activated protein kinase (AMPK, 2531; Cell Signaling), AMPKα (2532; Cell Signaling), Perilipin 5 (PA1-46215; Thermo Fisher Scientific), translocase of outer mitochondria membrane 70 (TOM70, 65675; Cell Signaling), cytochrome c oxidase subunit 4 (COXIV, 4844; Cell Signaling), and calnexin (208880; Merck, Rahway, NJ, USA). ..

    Article Title: Targeting GHSR-1α with silibinin to enhance mitophagy and prevent tubular injury in diabetic kidney disease.
    Article Snippet: Diabetic kidney disease (DKD) remains a leading cause of global renal failure, with tubular injury driven by mitochondrial dysfunction representing a critical yet therapeutically unaddressed mechanism.. Although the flavonolignan silibinin has demonstrated renoprotective potential, its precise molecular targets and mechanisms in DKD have remained unclear.. In this study, we combined integrated computational docking, biophysical assays, and comprehensive in vivo and in vitro models to investigate silibinin's interaction with the growth hormone secretagogue receptor-1α (GHSR-1α).

    Incubation:

    Article Title: Metformin drives HIF-1α–mediated dual metabolic reprogramming to enhance γδ T cell therapy in triple-negative breast cancer
    Article Snippet: Samples were resolved on 10% SDS–polyacrylamide gels and electrotransferred to 0.45-μm nitrocellulose membranes (IPVH00010, Merck millipore). .. The membrane was blocked with 5% non-fat milk in TBST (JXF003, Jingxin Bio) for 1 h at room temperature and subsequently incubated overnight at 4 °C with primary antibodies targeting phospho-AMPKα (2535 T, CST), AMPKα (2793S, CST), HIF-1α (2178S, CST), and β-actin (4970S, CST). .. The membrane was then subjected to three consecutive washes with TBST for 5 min, incubated for 1 h at room temperature with Anti-rabbit IgG HRP-linked Antibody (7074S, CST) or goat anti-mouse IgG HRP (abs20039, absin), washed three times in TBST for 5 min, and detected using the Omni-ECLTM Femto Light Chemiluminescence Kit (SQ201, EpiZyme).

    Western Blot:

    Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice
    Article Snippet: Densitometry was analyzed using ImageLab software (V6.0.1; Bio-Rad). .. Immunoblotting was performed with antibodies against lysocardiolipin acyltransferase 1(LCLAT1, PA5-25627; Thermo Fisher Scientific), PGC-1α (ab191838; Abcam, Cambridge, UK), MFN2 (PA5-118059; Thermo Fisher Scientific), ANP (sc-18811; Santa Cruz, Dallas, TX, USA), tumor necrosis factor alpha (TNFα, 3707; Cell Signaling, Danvers, MA, USA), phosphorylated adenosine monophosphate-activated protein kinase (AMPK, 2531; Cell Signaling), AMPKα (2532; Cell Signaling), Perilipin 5 (PA1-46215; Thermo Fisher Scientific), translocase of outer mitochondria membrane 70 (TOM70, 65675; Cell Signaling), cytochrome c oxidase subunit 4 (COXIV, 4844; Cell Signaling), and calnexin (208880; Merck, Rahway, NJ, USA). ..

    Pyrolysis Gas Chromatography:

    Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice
    Article Snippet: Densitometry was analyzed using ImageLab software (V6.0.1; Bio-Rad). .. Immunoblotting was performed with antibodies against lysocardiolipin acyltransferase 1(LCLAT1, PA5-25627; Thermo Fisher Scientific), PGC-1α (ab191838; Abcam, Cambridge, UK), MFN2 (PA5-118059; Thermo Fisher Scientific), ANP (sc-18811; Santa Cruz, Dallas, TX, USA), tumor necrosis factor alpha (TNFα, 3707; Cell Signaling, Danvers, MA, USA), phosphorylated adenosine monophosphate-activated protein kinase (AMPK, 2531; Cell Signaling), AMPKα (2532; Cell Signaling), Perilipin 5 (PA1-46215; Thermo Fisher Scientific), translocase of outer mitochondria membrane 70 (TOM70, 65675; Cell Signaling), cytochrome c oxidase subunit 4 (COXIV, 4844; Cell Signaling), and calnexin (208880; Merck, Rahway, NJ, USA). ..

    Aqueous Normal-phase Chromatography:

    Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice
    Article Snippet: Densitometry was analyzed using ImageLab software (V6.0.1; Bio-Rad). .. Immunoblotting was performed with antibodies against lysocardiolipin acyltransferase 1(LCLAT1, PA5-25627; Thermo Fisher Scientific), PGC-1α (ab191838; Abcam, Cambridge, UK), MFN2 (PA5-118059; Thermo Fisher Scientific), ANP (sc-18811; Santa Cruz, Dallas, TX, USA), tumor necrosis factor alpha (TNFα, 3707; Cell Signaling, Danvers, MA, USA), phosphorylated adenosine monophosphate-activated protein kinase (AMPK, 2531; Cell Signaling), AMPKα (2532; Cell Signaling), Perilipin 5 (PA1-46215; Thermo Fisher Scientific), translocase of outer mitochondria membrane 70 (TOM70, 65675; Cell Signaling), cytochrome c oxidase subunit 4 (COXIV, 4844; Cell Signaling), and calnexin (208880; Merck, Rahway, NJ, USA). ..

    Bioprocessing:

    Article Title: Dysregulation of a novel autophagosome-mitochondria contact contributes to autophagy dysfunction and neurodegeneration in tauopathy
    Article Snippet: .. Sources of antibodies or reagents are as follows: polyclonal antibodies against LC3 (Cell Signaling Technology, Cat# 2775), AMPKα (Cell Signaling Technology, Cat# 2532), p62/SQSTM1 (Abnova, Cat# H00008878-M01), NeuN (Millipore/Sigma, Cat# ABN78), TBC1D15 (Abcam, Cat# ab121396), Tau5 (DAKO, Cat# A0024), and synaptophysin/SYP (Abcam, Cat# ab32127); monoclonal antibodies against phospho-AMPKα (Cell Signaling Technology, Cat# 2535), AT8 (ThermoFisher Scientific, Cat# MN1020), syntaxin 1/STX1 (Santa Cruz Biotechnology, Cat# sc-12736), Rab7 (Sigma, Cat# R8779), GAPDH (Sigma, Cat# CB1001), TOM20 (Abcam, Cat# ab186734), mCherry (Takara, Cat# 632543), and Alexa fluor 488- (Cat# A-11017; Cat# A-11070), 546- (Cat# A-11018; Cat# A-11071), and 647- (Cat# A-21237; Cat# A-21246) conjugated secondary antibodies (Invitrogen); 10-NCP (VWR, Cat# 80017-188), trehalose (Cat# T0167), cycloheximide (Cat# 01810), rotenone (Cat# 557368), antimycin A (Cat# 1397-94-0), AICAR (Cat# A9978), CC (Cat# 171264), epoxomicin (Cat# 324800), CID 1067700 (Cat# SML0545), DMSO (Cat# D2650), and Sarkosyl (Cat# L9150) (Sigma); TBC1D15 shRNA (Cat# sc-154093-SH), Fis1 shRNA (Cat# sc-60644-SH), and control shRNA (Cat# sc-108060) (Santa Cruz Biotechnology); MitoView TM Green (Cat# 70054) (biotium); LentiBrite RFP-LC3 Lentiviral Biosensor (Cat# 17-10143) (Sigma). .. The constructs encoding GFP-LC3, DsRed-Mito, and mRFP-LC3 were prepared as we previously described ( , , , ). mCherry-TBC1D15 and mCherry-TBC1D15 D397A were from Vector Biolabs.

    shRNA:

    Article Title: Dysregulation of a novel autophagosome-mitochondria contact contributes to autophagy dysfunction and neurodegeneration in tauopathy
    Article Snippet: .. Sources of antibodies or reagents are as follows: polyclonal antibodies against LC3 (Cell Signaling Technology, Cat# 2775), AMPKα (Cell Signaling Technology, Cat# 2532), p62/SQSTM1 (Abnova, Cat# H00008878-M01), NeuN (Millipore/Sigma, Cat# ABN78), TBC1D15 (Abcam, Cat# ab121396), Tau5 (DAKO, Cat# A0024), and synaptophysin/SYP (Abcam, Cat# ab32127); monoclonal antibodies against phospho-AMPKα (Cell Signaling Technology, Cat# 2535), AT8 (ThermoFisher Scientific, Cat# MN1020), syntaxin 1/STX1 (Santa Cruz Biotechnology, Cat# sc-12736), Rab7 (Sigma, Cat# R8779), GAPDH (Sigma, Cat# CB1001), TOM20 (Abcam, Cat# ab186734), mCherry (Takara, Cat# 632543), and Alexa fluor 488- (Cat# A-11017; Cat# A-11070), 546- (Cat# A-11018; Cat# A-11071), and 647- (Cat# A-21237; Cat# A-21246) conjugated secondary antibodies (Invitrogen); 10-NCP (VWR, Cat# 80017-188), trehalose (Cat# T0167), cycloheximide (Cat# 01810), rotenone (Cat# 557368), antimycin A (Cat# 1397-94-0), AICAR (Cat# A9978), CC (Cat# 171264), epoxomicin (Cat# 324800), CID 1067700 (Cat# SML0545), DMSO (Cat# D2650), and Sarkosyl (Cat# L9150) (Sigma); TBC1D15 shRNA (Cat# sc-154093-SH), Fis1 shRNA (Cat# sc-60644-SH), and control shRNA (Cat# sc-108060) (Santa Cruz Biotechnology); MitoView TM Green (Cat# 70054) (biotium); LentiBrite RFP-LC3 Lentiviral Biosensor (Cat# 17-10143) (Sigma). .. The constructs encoding GFP-LC3, DsRed-Mito, and mRFP-LC3 were prepared as we previously described ( , , , ). mCherry-TBC1D15 and mCherry-TBC1D15 D397A were from Vector Biolabs.

    Control:

    Article Title: Dysregulation of a novel autophagosome-mitochondria contact contributes to autophagy dysfunction and neurodegeneration in tauopathy
    Article Snippet: .. Sources of antibodies or reagents are as follows: polyclonal antibodies against LC3 (Cell Signaling Technology, Cat# 2775), AMPKα (Cell Signaling Technology, Cat# 2532), p62/SQSTM1 (Abnova, Cat# H00008878-M01), NeuN (Millipore/Sigma, Cat# ABN78), TBC1D15 (Abcam, Cat# ab121396), Tau5 (DAKO, Cat# A0024), and synaptophysin/SYP (Abcam, Cat# ab32127); monoclonal antibodies against phospho-AMPKα (Cell Signaling Technology, Cat# 2535), AT8 (ThermoFisher Scientific, Cat# MN1020), syntaxin 1/STX1 (Santa Cruz Biotechnology, Cat# sc-12736), Rab7 (Sigma, Cat# R8779), GAPDH (Sigma, Cat# CB1001), TOM20 (Abcam, Cat# ab186734), mCherry (Takara, Cat# 632543), and Alexa fluor 488- (Cat# A-11017; Cat# A-11070), 546- (Cat# A-11018; Cat# A-11071), and 647- (Cat# A-21237; Cat# A-21246) conjugated secondary antibodies (Invitrogen); 10-NCP (VWR, Cat# 80017-188), trehalose (Cat# T0167), cycloheximide (Cat# 01810), rotenone (Cat# 557368), antimycin A (Cat# 1397-94-0), AICAR (Cat# A9978), CC (Cat# 171264), epoxomicin (Cat# 324800), CID 1067700 (Cat# SML0545), DMSO (Cat# D2650), and Sarkosyl (Cat# L9150) (Sigma); TBC1D15 shRNA (Cat# sc-154093-SH), Fis1 shRNA (Cat# sc-60644-SH), and control shRNA (Cat# sc-108060) (Santa Cruz Biotechnology); MitoView TM Green (Cat# 70054) (biotium); LentiBrite RFP-LC3 Lentiviral Biosensor (Cat# 17-10143) (Sigma). .. The constructs encoding GFP-LC3, DsRed-Mito, and mRFP-LC3 were prepared as we previously described ( , , , ). mCherry-TBC1D15 and mCherry-TBC1D15 D397A were from Vector Biolabs.



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    Image Search Results


    CCR5 acts as a positive upstream regulator of the AMPK/ACC signaling under hypoxia-ischemia-mimicking conditions. (A–B) SH-SY5Y cells were transfected with control scramble (Scr) or CCR5 shRNA and cultured under hypoxia-ischemia-mimicking conditions. Representative western blots (A) and quantitative analyses (B) of phosphorylated AMPK (p-AMPK), total AMPK, phosphorylated ACC (p-ACC), total ACC, and the corresponding phosphorylation ratios were shown. (C–D) SH-SY5Y cells were transfected with empty vector (EV) or a CCR5 overexpression plasmid and cultured under the indicated conditions. Representative western blots (C) and quantitative analyses (D) of p-AMPK, AMPK, p-ACC, ACC, and the corresponding phosphorylation ratios were shown. (E–F) SH-SY5Y cells were treated with MVC and cultured under normal conditions or hypoxia-ischemia-mimicking conditions. Representative western blots (E) and quantitative analyses (F) of p-AMPK, AMPK, p-ACC, ACC, and the corresponding phosphorylation ratios were shown. β-Tubulin was used as a loading control. Data are presented as mean ± SD (n = 3). Statistical analyses were performed using one-way ANOVA followed by Fisher's LSD post hoc tests. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

    Journal: Neurotherapeutics

    Article Title: CCR5 protects against permanent brain ischemia by suppressing neuronal ferroptosis through AMPK/ACC signaling

    doi: 10.1016/j.neurot.2026.e01088

    Figure Lengend Snippet: CCR5 acts as a positive upstream regulator of the AMPK/ACC signaling under hypoxia-ischemia-mimicking conditions. (A–B) SH-SY5Y cells were transfected with control scramble (Scr) or CCR5 shRNA and cultured under hypoxia-ischemia-mimicking conditions. Representative western blots (A) and quantitative analyses (B) of phosphorylated AMPK (p-AMPK), total AMPK, phosphorylated ACC (p-ACC), total ACC, and the corresponding phosphorylation ratios were shown. (C–D) SH-SY5Y cells were transfected with empty vector (EV) or a CCR5 overexpression plasmid and cultured under the indicated conditions. Representative western blots (C) and quantitative analyses (D) of p-AMPK, AMPK, p-ACC, ACC, and the corresponding phosphorylation ratios were shown. (E–F) SH-SY5Y cells were treated with MVC and cultured under normal conditions or hypoxia-ischemia-mimicking conditions. Representative western blots (E) and quantitative analyses (F) of p-AMPK, AMPK, p-ACC, ACC, and the corresponding phosphorylation ratios were shown. β-Tubulin was used as a loading control. Data are presented as mean ± SD (n = 3). Statistical analyses were performed using one-way ANOVA followed by Fisher's LSD post hoc tests. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

    Article Snippet: Following electrophoretic separation, the proteins were transferred onto a nitrocellulose membrane, which was then blocked with 5% skimmed milk at room temperature for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies against HIF-1α (ABclonal Technology, A24002), CCR5 (Affinity Biosciences LTD, AF6339), Phospho-AMPKα (Thr172) (Beyotime, AA393), AMPKα (Proteintech, 10929-2-AP), Phospho-ACC (Proteintech, 29119-1-AP), ACC (Proteintech, 21923-1-AP), or GPX4 (Abmart, T56959 ), each diluted to a ratio of 1:1000 in 5% bovine serum albumin (BSA).

    Techniques: Transfection, Control, shRNA, Cell Culture, Western Blot, Phospho-proteomics, Plasmid Preparation, Over Expression

    AMPK inhibition completely abolishes CCR5-mediated GPX4 maintenance and anti-ferroptotic protection. (A–B) SH-SY5Y cells were treated with the AMPK inhibitor Compound C (COC, 10 μM) and cultured under hypoxia-ischemia-mimicking conditions. Representative western blots (A) and quantitative analyses (B) of phosphorylated AMPK (p-AMPK), total AMPK, phosphorylated ACC (p-ACC), total ACC, and the corresponding phosphorylation ratios were shown. (C–E) Under the same experimental conditions, representative western blots (C) and quantitative analyses (D) of CCR5 and GPX4 protein expression, together with MDA levels (E), were shown. β-Tubulin or β-actin were used as a loading control. Data are presented as mean ± SD (n = 3). Statistical analyses were performed using one-way ANOVA followed by Fisher's LSD post hoc tests. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

    Journal: Neurotherapeutics

    Article Title: CCR5 protects against permanent brain ischemia by suppressing neuronal ferroptosis through AMPK/ACC signaling

    doi: 10.1016/j.neurot.2026.e01088

    Figure Lengend Snippet: AMPK inhibition completely abolishes CCR5-mediated GPX4 maintenance and anti-ferroptotic protection. (A–B) SH-SY5Y cells were treated with the AMPK inhibitor Compound C (COC, 10 μM) and cultured under hypoxia-ischemia-mimicking conditions. Representative western blots (A) and quantitative analyses (B) of phosphorylated AMPK (p-AMPK), total AMPK, phosphorylated ACC (p-ACC), total ACC, and the corresponding phosphorylation ratios were shown. (C–E) Under the same experimental conditions, representative western blots (C) and quantitative analyses (D) of CCR5 and GPX4 protein expression, together with MDA levels (E), were shown. β-Tubulin or β-actin were used as a loading control. Data are presented as mean ± SD (n = 3). Statistical analyses were performed using one-way ANOVA followed by Fisher's LSD post hoc tests. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

    Article Snippet: Following electrophoretic separation, the proteins were transferred onto a nitrocellulose membrane, which was then blocked with 5% skimmed milk at room temperature for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies against HIF-1α (ABclonal Technology, A24002), CCR5 (Affinity Biosciences LTD, AF6339), Phospho-AMPKα (Thr172) (Beyotime, AA393), AMPKα (Proteintech, 10929-2-AP), Phospho-ACC (Proteintech, 29119-1-AP), ACC (Proteintech, 21923-1-AP), or GPX4 (Abmart, T56959 ), each diluted to a ratio of 1:1000 in 5% bovine serum albumin (BSA).

    Techniques: Inhibition, Cell Culture, Western Blot, Phospho-proteomics, Expressing, Control